Hemoglobin is a protein present in RBCs for exchange of gases with tissues. Blood is a specialized type of connective tissue in the form of fluid. It has two components called as plasma and cells.
- Plasma contains 12 coagulation factors. Serum is formed by the removal of these coagulation factors. Serum is collected in red color vacuotainer. It does not let the blood clot.
- The ratio of cells in the blood is RBCs, WBCs and Platelets as 500:1:30 respectively.
There are four methods for the determination of hemoglobin in the blood;
- Sahli’s method..Outdated
- Cyanmet Hb method..Updated
- Haden Haussen method
Sahli’s Method of Hemoglobin Determination:
- Sahli’s tube which is having red and yellow scales on two sides. Red scale is percentage scale and yellow scale is gram percentage or g/100ml scale.
- Heamometer which is having two standards.
- Sahli’s pipette.
- Error percentage is 3%.
- In sahli’s tube, take N/10 HCL(1/10th of the original HCL) up to 10th level of scale.
- In sahli’s pipette, take 0.02ml(20microleter) blood.
- Add blood from pipette into tube.
- HCL will cause the lysis of the blood cells and hemoglobin is released.
- Hb after combining with HCL, forms acid hematin which is of tan color.
- Put tube in the hemometer and continuously add drops of distilled water and shake with the stirrer until color matches. Then, take the reading.
Interpretation of the results: